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Efficient Generation of Germ Line Transmitting Chimeras ...

Conditioned ES cell medium significantly improves chimera production from B6-derived C2 ES cells. Blastocyst injection is the most common method for producing mouse ES cell chimeras. 8-cell or morula microinjections can be performed with the conventional technique routinely used for blastocysts, as well as by introducing a slit cut or hole in the zona pellucida using a glass needle,, piezo ...

Center for Animal Transgenesis & Germ Cell Research

Contact Information. The Center for Animal Transgenesis and Germ Cell Research of the School of Veterinary Medicine is a state of the art facility that offers a full line of embryological manipulation services, focused on, but not limited to, murine model systems. These services primarily enable the generation of genetically modified murine models,...

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In-cell Aggregation of a Polyglutamine-containing Chimera ...

In-cell Aggregation of a Polyglutamine-containing Chimera Is a step Process Initiated by the Flanking Sequence Article (PDF Available) in Journal of Biological Chemistry 282(50):36736-43 ...

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Application of Chimera grid to modelling cell ... - DeepDyve

Jan 10, 2007 · Read "Application of Chimera grid to modelling cell motion and aggregation in a narrow tube, International Journal for Numerical Methods in Fluids" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

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Application of Chimera grid to modelling cell ... - DeepDyve

Jan 10, 2007 · Read "Application of Chimera grid to modelling cell motion and aggregation in a narrow tube, International Journal for Numerical Methods in Fluids" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

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Chimaera | Definition of Chimaera at Dictionary

Chimaera definition, any fish of the family Chimaeridae, the male of which has a spiny clasping organ over the mouth. See more.

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Aggregation Chimeras | SpringerLink

In recent years, chimeras have been providing a powerful way to study mouse development in combination with invention and improvement of other techniques and materials, including embryonic stem (ES) cells and tetraploid embryos (3, 4).ES cells are pluripotent cell lines derived from late blastocyst-stage embryos, which are capable of differentiating into all derivatives of the primitive ...

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Production of chimeras by aggregation of embryonic stem ...

The production of mouse chimeras is a common step in the establishment of genetically modified animal strains. Chimeras also provide a powerful experimental tool for following cell behavior during ...

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Aggregation Chimeras: Combining ES Cells, Diploid, and ...

This aggregation combination is suitable for all uses described in Section 1.1.1. For the study of cell autonomy during the development of primitive ectoderm derivatives, mutant ES cell lines carrying haploinsufficient or X-chromosome-linked or dominant mutations, or .

Chimera (genetics) - Infogalactic: the planetary knowledge ...

The Inner Cell Mass of a diploid blastocyst for example can be used to make a chimera with another blastocyst of eight-cell diploid embryo; the cells taken from the inner cell mass will give rise to the primitive endoderm and to the epiblast in the chimera mouse. From this knowledge, ES cell contributions to chimeras have been developed.

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Inhibition of protein aggregation in vitro and in vivo by ...

Sep 05, 2006 · Small organic molecules termed osmolytes are harnessed by a variety of cell types in a wide range of organisms to counter unfavorable physiological conditions that challenge protein stability and function. Using a well characterized reporter system that we developed to allow in vivo observations, we have explored how the osmolyte proline influences the stability and aggregation of a model ...

(PDF) Aggregation Chimeras: Combining ES Cells, Diploid ...

The chimera competency of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in mice and embryo-embryo aggregation in pigs has recently been utilized for generating specific organs ...

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CHIMERA | definition in the Cambridge English Dictionary

chimera meaning: 1. a hope or dream that is extremely unlikely ever to come true: 2. an organism that contains cells or tissues from two or more different species: . Learn more.

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Definition of Chimera - MedicineNet

Chimera: In medicine, a person composed of two genetically distinct types of cells. Human chimeras were first discovered with the advent of blood typing when it was found that some people had more than one blood type. Most of them proved to be "blood chimeras" -- non-identical twins who shared a blood supply in the uterus.Those who were not twins are thought to have blood cells from a twin ...

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In-cell Aggregation of a Polyglutamine-containing Chimera ...

In-cell Aggregation of a Polyglutamine-containing Chimera Is a step Process Initiated by the Flanking Sequence Article (PDF Available) in Journal of Biological Chemistry 282(50):36736-43 ...

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Contributions of Mammalian Chimeras to ... - cell

Experimental chimeras provide key insights into mammalian developmental mechanisms and offer a resource for interrogating the fate potential of various pluripotent stem cell states. Mascetti and Pedersen highlight the applications and current limitations presented by intra- and inter-species chimeras and consider their future contribution to the stem cell field.

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Chimaera | Definition of Chimaera at Dictionary

Chimaera definition, any fish of the family Chimaeridae, the male of which has a spiny clasping organ over the mouth. See more.

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CiteSeerX — Effects of the lethal yellow (A*) mutation in ...

CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The Ay allele is a recessive lethal mutation at the mouse agouti locus, which results in embryonic death around the time of implantation. In the heterozygous state, Ay produces several dominant pleiotropic effects, including an increase in weight gain and body length, a susceptibi-lity to hepatic, pulmonary and ...

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Production of chimeras by aggregation of embryonic stem ...

Aggregation, on the other hand, requires no specialized equipment and is inexpensive and relatively easily learned, but it saces the ability to select ES cells individually. This can result in the reduction of chimera quality, particularly for higher-passage or otherwise problematic ES cells 12. These differences aside, the choice of ...

Generation of Chimeric Rhesus Monkeys - ScienceDirect

Parentage Analysis of Twin Chimeras Derived by Four-Cell Embryo Aggregation, Related to Table 1 (A) Representative STR loci for EA-f2 and -f3 (embryo aggregation chimera twins). Parental alleles representing sperm donor male #5, egg donor #11, and egg donor #12 are highlighted with blue, red, and green fonts, respectively.

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